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bgl ii bam hi fragment  (TaKaRa)


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    Structured Review

    TaKaRa bgl ii bam hi fragment
    Bgl Ii Bam Hi Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+hi+bgl+ii+fragment/Bgl+II/pmc06492720-60-4-16
    Average 97 stars, based on 3916 article reviews
    bgl ii bam hi fragment - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Fluorescence:

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Construct:

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Cloning:

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Expressing:

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Clone Assay:

    Article Title: Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics
    Article Snippet: To generate pIN-1, pIRESHyg3 (Clontech) was digested with Xho I/ Nhe I and the IRES-Hyg fragment was cloned into the Xba I/ Sal I site of plox66luc, downstream of the luciferase ORF. .. The gpt ORF was removed from pBSgpt [ ] as a Bam HI/ Bgl II fragment and cloned into the Bgl II site of pGL3-Basic (Clontech) to make pGL3gptluc. ..

    Article Title: Functional Interaction Map of Lyssavirus Phosphoprotein: Identification of the Minimal Transcription Domains
    Article Snippet: .. First the EGFP gene (Clontech) was amplified with primers FUS-GFP 5′ (5′ CGGGATCCATGGGTAAAGGAGAAGAAC 3′) and FUS-GFP 3′ (5′ GAAGATCTTATTTGTATAGTTCATCCATGCC 3′) and the Bam HI- Bgl II fragment was cloned into the Bam HI site of pACTII (Clontech). ..

    Article Title: Macrophage Plasma Membrane Cholesterol Contributes to Brucella abortus Infection of Mice
    Article Snippet: .. Plasmid pMAW114, which encodes green fluorescence protein (GFP), was constructed by cloning the Bam HI- Bgl II fragment from the pQBI63 (GFP expression vector; TAKARA, Tokyo, Japan) into Bam HI- and Bgl II-cleaved pBBR1MCS-2. pMAW114 (GFP + ) was introduced into 544 (wild type) and Ba598 (Δ virB4 ), and the derivatives were designated Ba600 (wild-type GFP + ) and Ba604 (Δ virB4 GFP + ), respectively ( 33 ). .. BALB/c mice carrying the genetic mutation for NPC1 were obtained from The Jackson Laboratory (Bar Harbor, Maine) ( 25 ).

    Amplification:

    Article Title: Functional Interaction Map of Lyssavirus Phosphoprotein: Identification of the Minimal Transcription Domains
    Article Snippet: .. First the EGFP gene (Clontech) was amplified with primers FUS-GFP 5′ (5′ CGGGATCCATGGGTAAAGGAGAAGAAC 3′) and FUS-GFP 3′ (5′ GAAGATCTTATTTGTATAGTTCATCCATGCC 3′) and the Bam HI- Bgl II fragment was cloned into the Bam HI site of pACTII (Clontech). ..



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